Larger Field-of-View: New large DMD chip combined with interchangeable front tube optics (1x & 2x*) enables larger field-of-view without comprising resolution and power, * 2X front tube lens sold separately;
Market-leading Speed: Increased maximum frame rate means better temporal resolution for advanced physiologically-relevant experiments and virtually simultaneous 2-colour illumination of distinct ROIs;
Faster uploading time: Faster uploading time, up to 4ms uploading speed per frame, allows the Polygon1000 to perform real-time pattern illumination for closed-loop experiments;
Flexibility: Enable to coupled into the infinity space of any microscope model with Mightex’s microscope-specific adapters, MPI multi-port illuminator, and Nikon’s LAPP modular illumination system.
State-of-the-Art Illumination Technology
The Polygon uses digital mirror device (DMD) technology to illuminate multiple regions simultaneously. A DMD is composed of hundreds of thousands of micro-mirrors that can be individually turned on to reflect light onto the sample. Thus, you can control each mirror to control the area(s) of illumination and create any number of different sized patterns. The Polygon DMD illuminator can be mounted into the infinity-path of any microscope.
A. Neuroscience Optogenetics
Optogenetics has advanced the field of neuroscience by providing a precise method for manipulating neuronal activity. The ability to perform cellular-resolution optogenetics enables researchers to stimulate select optogenetic-expressing neurons (e.g. ChR2, NpHr, Chrimson etc.) in slice or culture during electrophysiological recordings or calcium imaging to map neural circuitry.
Optogenetic toolkits have expanded to offer cell biologists unprecedented spatiotemporal molecular and cellular control. From cell migration to gene regulation or developmental processes, cell biologists now have the ability to elicit specific biological responses with an incredible amount of precision in specific cells within organisms or subcellular regions of individual cells using optogenetics.
Advances in fluorescent protein technology have made photoactivation techniques broadly available in the cell biology lab. By using these techniques, researchers can fluorescently highlight/label specific protein populations in the cell such that their movement and behavior can be tracked over time. Also, photoactivation methods can be applied at different scales, allowing researchers to track proteins within cells, or alternatively to track cells within tissues.
Many techniques have been developed to manipulate the different properties of the cellular microenviroment. Recently, the advent of light-induced biochemical compounds has allowed scientists to define the biochemical and structural features of cellular matrices by photo micropatterning and photolithography. Scientists can precisely define the spatial distribution of UV light to create unique cellular microenvironments without any use of masks or physical contact with the substrate.
Micro-Fluidic devices are made by photo-patterning thin film precursor materials on a substrate to create microscopic channels, chambers, and even flow-controlling mechanisms. Small amounts of fluids containing reactive chemicals or other substances can then flow through the micro-channels and mix in the micro-chambers of the device and react with other substances that may be deposited in the micro-channels.