Mightex BioLED Analog and Digital I/O Control Module is a system-level control solution to be used in conjunction with the BioLED Manual/Analog Controlled LED Controllers and with the Polygon Patterned Illuminator. It greatly extends the flexibility and ease of system-level set-up when working with Mightex’s Polygon systems. The I/O Control Module features four (4) independent channels. Each channel has an external trigger input (TTL), an analog voltage output (0-5V) and a digital output (LVTTL). All I/O connectors are BNC-type to work seamlessly with laboratory equipment. Intensity waveform of up to four (4) high-power LED light sources is controlled by the I/O Control Module1, each can output light at a different wavelength (i.e. color). Up to thirty-two (32) different waveforms can be programmed onto the I/O Control Module via USB or RS232. Each waveform can be associated with any one of the four LED’s to form a catalog of up to 128 unique Color/Waveform pairs. The I/O Control Module can be synchronized with Polygon Patterned Illuminator. Each illumination pattern can be synchronized with one of the 128 Color/Waveform pairs, and one can generate a complex sequence of illumination Pattern/Color/Waveform combinations quickly and precisely. The I/O Control Module thus provides a convenient means to simultaneously achieve spatial, spectral, and temporal control of the most advanced illumination system for optogenetics and other experiments.